Bacterial adherence to polystyrene: a replica method of screening for bacterial hydrophobicity.
نویسنده
چکیده
A simple replica method is described for the rapid identification of colonies of bacteria which adhere to polystyrene. A correlation was found between the adherence of bacterial strains to polystyrene and cell surface hydrophobicity, suggesting the use of this technique in screening for cell surface mutants and in the isolation of hydrophobic bacteria from nature.
منابع مشابه
Hydrophobicity effect on oil degradation by two marine bacterial strains Alcanivorax borkumensis and Thalassolituus oleivorans
Variations on hydrophobicity were monitored in two marine obligate hydrocarbonoclastic bacteria: Alcanivorax borkumensis SK2T and Thalassolituus oleivoras MIL-1T. These strains were inoculated, separately in ONR7a mineral medium with different concentration of sodium acetate. During 10 days measurements of cellular abundance and cellular hydrophobicity (capacity to adhere at polystyrene) were c...
متن کاملThe Relationship between Cell Surface Hydrophobicity and Antibiotic Resistance of Streptococcal Strains Isolated from Dental Plaque and Caries
Objective Bacterial adhesion is governed by specific and nonspecific interactions such as hydrophobicity. Hydrophobic interactions play a role in the adherence of microorganisms to a wide variety of surfaces and facilitate biofilm formation due to bacterial adhesion. In this article the relation between cell surface hydrophobicity and antibiotic resistance was studied. Materials and Methods ...
متن کاملBacterial cell surface hydrophobicity properties in the mediation of in vitro adhesion by the rabbit enteric pathogen Escherichia coli strain RDEC-1.
The role of hydrophobicity in the attachment of enteropathogens to gastrointestinal mucosa is controversial. In vitro binding of Escherichia coli RDEC-1 to rabbit intestine is dependent on the expression of pili. We examined in vitro adherence of piliated RDEC-1 after altering either the hydrophobicity of the organisms, the hydrophobicity of the substrate for attachment, or the surface tension ...
متن کاملEffects of DNP on the cell surface properties of marine bacteria and its implication for adhesion to surfaces.
The effect of 2, 4-dinitrophenol (DNP) on the extracelluar polysaccharides (EPS), cell surface charge, and the hydrophobicity of six marine bacterial cultures was studied, and its influence on attachment of these bacteria to glass and polystyrene was evaluated. DNP treatment did not influence cell surface charge and EPS production, but had a significant effect on hydrophobicity of both hydrophi...
متن کاملQuantitative Bacterial Micro-Assay for Rapid Diagnosis of Galactosemia: Application in Galactosemia Neonatal Screening
In the present study a new economic and rapid bacterial micro-assay for simultaneous detection and quantitative measurement of serum galactose was developed. Analysis of the standard curve showed a linearity range for galactose from 2 mg/dL to 180 mg/dL with a regression equation of Y = 0.013X ? 0.083; R² = 0.962. The advantage of the method is its ability to measure serum galactose quantitativ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 42 2 شماره
صفحات -
تاریخ انتشار 1981